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Part:BBa_K4105001

Designed by: Symbat Alimbek   Group: iGEM21_Yonsei_Korea   (2021-10-12)


DNAzyme

DNAzyme specifically cleaves the target mif23 gene found in Magnaporthe oryzae. Specifically, it recognizes the YTGC (Y=T/C) region of the gene and cleaves at T residue, producing several cleaved products. This 100 base pairs long DNAzyme consists of 3 subregions: a left binding arm, a catalytic loop region, and a right binding arm. Two binding arms assist DNAzyme to hybridize with the target sequence to carry out the loop catalysed cleavage mechanism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//dna
//dna/dnazyme
//dna/nucleotide
Parameters
functionDNA cleaving enzyme